Description
Principle of the Assay: This kit was based onindirect enzyme-linked immune-sorbent assay technology. OVAwas pre-coated onto 96-well plates. And the HRP conjugated anti-mouse OVA IgG antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the OVA IgG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of OVA IgG can be calculated